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Tools and Techniques in Biomolecular Science

Edited by Aysha Divan and Janice Royds

Publication Date - May 2013

ISBN: 9780199695560

528 pages
10.4 x 7.7 inches

An in-depth look at the theoretical concepts and experimental details behind modern technologies


Tools and Techniques in Biomolecular Science reviews the theoretical concepts and experimental details underpinning the broad range of modern technologies that are currently being used to advance our understanding of the biomolecular sciences.

Each chapter focuses on the principles, applications, and limitations of a different technology. Fostering active engagement with the techniques presented, each chapter uses worked examples and problems to encourage students to critically appraise scientific data and select the most appropriate methods when designing an experimental strategy to undertake their own research.


* Provides a clear overview of contemporary biomolecular techniques, making it the ideal resource for students and early-stage researchers

* Uses examples throughout to illustrate how each technique is applied, and shows how the data yielded can be analyzed and understood

* Emphasizes the pros and cons of the techniques presented, helping students to evaluate the experimental options at their disposal

* Worked examples encourage students to become confident with data handling--a central part of any experimental research

* A Companion Website provides a range of additional resources

Table of Contents

    1. Gene Cloning Essentials, David Hornby and Qaiser I. Sheikh

    2. PCR, Martin Evison

    3. DNA Mutagenesis, Sarah E. Deacon and Michael J. McPherson

    4. DNA Sequencing, Tania Slatter and Alison Fitches

    5. Measuring DNA/Protein Interactions, Luis Acevedo, Ana Sanz, Paul Labhart, and Mary Anne Jelinek

    6. RNA Interference Technology, Gavin Batman, Thomas Walker, and Ian Hampson

    7. Recombinant Protein Expression, Michael Harrison and Michael J. McPherson

    8. Protein Purification, Iain Manfield

    9. Antibody Technology, Gavin Allsop and John Colyer

    10. Measuring Protein-Protein Interactions: Qualitative Approaches, Johanna M. Avis and Sanjay Nilapwar

    11. Measuring Protein-Protein Interactions: Quantitative Approaches, Johanna M. Avis and Sanjay Nilapwar

    12. Structural Analysis of Proteins: X-ray Crystallography, Thomas Edwards, Arwen Pearson, Gary Thompson, Arnout Kalverda, Oliver Farrance, David Brockwell, and Gareth Morgan

    13. Mass Spectrometry, James R. Ault and Alison E. Ashcroft

    14. Proteomic Analysis, Richard Unwin

    15. Culturing Mammalian Cells, Geoffrey J. Pilkington, Suzanne M. Birks, and Laura K. Donovan

    16. Flow Cytometry, John Lawry

    17. Bioimaging: Light and Electron Microscopy, Gareth Howell and Kyle Dent

    18. Histopathology in Biomolecular Research, Noelyn Hung and Tania Slatter

    19. Mouse Models in Scientific Research, Brian Corbett and Jeannie Chin

    20. Mathematical Models in Biomolecular Sciences, José A. García

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