Tools and Techniques in Biomolecular Science

Description

Tools and Techniques in the Biomolecular Sciences reviews a broad range of modern technologies, explaining the theoretical principles of each technology, their applications and limitations, and how to understand and analyse the data a particular technique generates.

• Provides a clear overview of a wide range of contemporary biomolecular techniques, making it the ideal resource for students and early-stage researchers
• Uses examples throughout to illustrate how each technique is applied, and shows how the data yielded can be analysed and understood
• Emphasises the pros and cons of the techniques presented, helping readers to evaluate the experimental options at their disposal
• Worked examples and associated data sets encourage the reader to become confident with data handling - a central part of any experimental research
• An Online Resource Centre provides a range of additional resources to augment the printed text

About the Author(s)

Edited by Aysha Divan, Lecturer in the Faculty of Biological Sciences at the University of Leeds, and Janice Royds, Lecturer in the Department of Pathology at the University of Otago, New Zealand

Dr Aysha Divan is a lecturer in the Faculty of Biological Sciences at the University of Leeds, where her laboratory-based research includes tumour biology, and her pedagogical research examines postgraduate-level communication and technical skills training in the biosciences.

Dr Janice Royds is a lecturer in the Department of Pathology at the University of Otago, New Zealand. Her broad ranging biomedical research interests relate to cancer biology, while her extensive teaching experience at both undergraduate and postgraduate level include pathology, pharmacology, and the molecular biology of cancer.

Table of Contents

1:Gene Cloning Essentials David Hornby & Qaiser I Sheikh
2:PCR Martin Evison
3:DNA Mutagenesis Sarah E. Deacon & Michael J. McPherson
4:DNA Sequencing Tania Slatter & Alison Fitches
5:Measuring DNA/Protein Interactions Luis Acevedo, Ana Sanz, Paul Labhart & Mary Anne Jelinek
6:RNA Interference Technology Gavin Batman, Thomas Walker & Ian Hampson
7:Recombinant Protein Expression Michael Harrison & Michael J. McPherson
8:Protein Purification Iain Manfield
9:Antibodies as Research Tools Gavin Allsop & John Colyer
10:Measuring Protein-Protein Interactions: Qualitative Approaches Johanna M. Avis & Sanjay Nilapwar
11:Measuring Protein-Protein Interactions: Quantitative Approaches Johanna M. Avis & Sanjay Nilapwar
12:Structural Analysis of Proteins: X-ray Crystallography, NMR, AFM, and CD spectroscopy Thomas Edwards, Arwen Pearson, Gary Thompson, Arnout Kalverda, Oliver Farrance, David Brockwell & Gareth Morgan
13:Mass Spectrometry James R. Ault and Alison E. Ashcroft
14:Proteomic Analysis Richard Unwin
15:Culturing Mammalian Cells Geoffrey J Pilkington, Suzanne M. Birks & Laura K. Donovan
16:Flow Cytometry John Lawry
17:Bioimaging: Light and Electron Microscopy Gareth Howell & Kyle Dent
18:Histopathology in Biomolecular Research Noelyn Hung & Tania Slatter
19:Mouse Models in Bioscience Research Brian Corbett & Jeannie Chin
20:Mathematical Models in Biomolecular Sciences Josie Athens

Reviews

The book provides a valuable laboratory resource, especially for students trying to understand the theory and background of their experiments. - Professor Bruce Baguley and Dr Euphemia Leung, Auckland Cancer Society Research Centre