Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules

Description

This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a comprehensive way and gives examples of applications in various fields of biophysics and biochemistry.

• First up-to-date book covering the whole of the field
• Authors have pioneered many of the methods described
• Material presented immediately relevant to research of the target audience
• Extensive coverage of the literature concerning methods as well as applications
• Contains mathematical and web-related appendices

About the Author(s)

Dmitri I. Svergun, Group Leader and Senior Scientist, European Molecular Biology Laboratory, Hamburg, Michel H. J. Koch, Department of Chemistry, University of Leuven, Peter A. Timmins, Long-term Visitor, Institut Laue-Langevin, Grenoble, Roland P. May, Long-term Visitor, Institut Laue-Langevin, Grenoble, Dmitri I. Svergun, Group Leader and Senior Scientist, European Molecular Biology Laboratory, Hamburg, Michel H. J. Koch, Department of Chemistry, University of Leuven, Peter A. Timmins, Long-term Visitor, Institut Laue-Langevin, Grenoble, and Roland P. May, Long-term Visitor, Institut Laue-Langevin, Grenoble

Dmitri I.Svergun: MSc in Solid state physics (1980), Physics Department, Moscow State University, Russia; PhD in Physics and Mathematics (1982) and Dr. of Science (1997), Institute of Crystallography, Moscow, Russia; Engineer, Researcher, Senior Scientist at the Institute of Crystallography, Russian Academy of Sciences, Moscow (1980 - 2008); Guest scientist, GKSS Research Centre, Geesthacht, Germany (1990-1991); Visitor, Staff Scientist, Group Leader, at the European Molecular Biology Laboratory Hamburg Outstation (1991-present).

Michel, H.J. Koch: PhD in chemistry, University of Louvain (1970), Neutron scattering on biological macromolecules in solution (1974-1976). Consultant (1975-1976), Group leader at the European Molecular Biology Laboratory outstation at DESY, Hamburg (1977-2006), Visiting professor at the Faculty of Engineering and Natural Science, Sabanci University, Turkey (2008-2009) and the Chemistry Department, University of Leuven (2006 - present).

Roland May: Diploma in Physics (Technische Universität München); Thesis work at the Max-Planck Institut für Biochemie, Martinsried; PhD Technische Universität München 1978; scientist in the Large-Scale Structures group of the Institut Laue-Langevin, Grenoble until 2008; retired.

Peter Timmins: BSc in Chemistry (1967), MSc in Crystallography (1968) and PhD in Crystallography (1972) - Birkbeck College, University of London. Instrument Scientist, Institut Laue-Langevin, 1975 - 1993, Head, Large Scale Structures Group, Institut Laue-Langevin, 1993 - 2010. Since 2010, retired.

Table of Contents

Introduction
PART I. THEORY AND EXPERIMENT
1:Basics of small-angle scattering
2:X-ray and neutron scattering instruments
3:Experimental practice and data processing
PART II. DATA ANALYSIS METHODS
4:Monodisperse systems
5:Polydisperse and interacting systems
PART III. BIOLOGICAL APPLICATIONS OF SOLUTION SAS
6:Static structral studies
7:Kinetic and perturbation studies
8:Analysis of interparticle interactions
9:SAS in multidisciplinary studies
Conclusions and future outlook
Appendix 1: Waves, complex number, Fourier transforms, convolution, correlation
Appendix 2: Spherical harmonics formalism
Appendix 3: Interactions between spherical molecules
Appendix 4: List of Web resources
Introduction
PART I. THEORY AND EXPERIMENT
1:Basics of small-angle scattering
2:X-ray and neutron scattering instruments
3:Experimental practice and data processing
PART II. DATA ANALYSIS METHODS
4:Monodisperse systems
5:Polydisperse and interacting systems
PART III. BIOLOGICAL APPLICATIONS OF SOLUTION SAS
6:Static structral studies
7:Kinetic and perturbation studies
8:Analysis of interparticle interactions
9:SAS in multidisciplinary studies
Conclusions and future outlook
Appendix 1: Waves, complex number, Fourier transforms, convolution, correlation
Appendix 2: Spherical harmonics formalism
Appendix 3: Interactions between spherical molecules
Appendix 4: List of Web resources

Reviews

"This book is a masterly treatment by four authors with many decades of experience of SAXS and SANS and who are from world class synchrotron X-ray and neutron research facilities. From the basics and on through the instrumentation and data analysis descriptions, as well as numerous research themes and examples, this book will be invaluable to newcomers and be useful to the specialists. It also should be read in detail by structural biology researchers whether principally from crystallography, bio NMR, EM or bioinformatics. It's surely going to be a classic book." - John Helliwell, University of Manchester

"Small angle X-ray and neutron scattering from solutions of biological macromolecules is an essential book covering the theory, experiment, data analysis, and applications of small-angle scattering (SAS) ... No laboratory interested in SAS should be without a copy and it provides a good background to the capabilities, limitations, and practical application of the technique. The authors should be commended and the book is strongly recommended." - Crystallography Reviews