Elliott & Elliott: Biochemistry and Molecular Biology 4e
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The human genome
The polymerase chain reaction
Saiki, R. K., et al. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487-91 [DOI: 10.1126/science.2448875] [PubMed: 2448875].
Detailed account of the technology of the PCR reaction.
Melton, D. W. (1994). Gene targeting in the mouse. BioEssays, 16, 633-8 [DOI: 10.1002/bies.950160907] [PubMed: 7980488].
Describes the technology of obtaining knockout mice and discusses its application to study of human diseases.
Stoughton, R. B. (2005). Application of DNA microarrays in biology. Annu. Rev. Biochem., 74, 53 [DOI: 10.1146/annurev.biochem.74.082803.133212].
Topol, E. J., Murray, S. H. and Frazer, K. A. (2007). The genomics goldrush. J. Amer. Med. Assoc., 298, 218-21 [DOI: 10.1001/jama.298.2.218].
Deals with the medical potential of using microarray analysis of single nucleotide polymorphisms to make genome-wide profiles of individual patients. The goldrush refers to the enormous activity in this field.
Karnoub, A. E., Dash, A. B., Vo, A. P., Sullivan, A., Brooks, M. W., Bell, G. W., Richardson, A. L., Polyak, K., Turbo, R. and Weinberg, R. A. (2007). Mesenchymal stem cells within a tumour stroma promote breast cancer metastasis. Nature, 449, 557-65 [DOI: 10.1038/nature06188].
Rossant, J. (2007). The magic brew. Nature, 448, 260-2 [DOI: 10.1038/448260a] [PubMed: 17637646].
The brew is the cocktail of transcription factors used to induce adult skin cells to become plutipotent. A News and Views article summarizing three reports on this subject.
Seandel, M. et al. (2007). Generation of functional multipotent adult stem cells from GPR125+ germline progenitors. Nature, 449, 346-50 [DOI: 10.1038/nature06129] [PubMed: 17882221].
Reports of the isolation from testis of pluripotent stem cells with potential for male therapy.
Morgan, R. A. and Anderson, W. E. (1993). Human gene therapy. Annu. Rev. Biochem., 62, 191-217 [DOI: 10.1146/annurev.bi.62.070193.001203].
Deals with the candidate diseases, gene-transfer methods, treatment of adenosine deaminase deficiency, and concludes with safety and ethical questions.
Sunyaer, S. R., et al. (2000). SNP frequencies in human genes: an excess of rare alleles and differing modes of selection. Trends Genet., 16, 335-7 [DOI: 10.1016/S0168-9525(00)02058-8].
(SNP = single nucleotide polymorphism.)
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A special News and Views article describing how Dolly was cloned by nuclear transplantation including discussion of the potential use of stem cell technology for growth of organ transplants, the ethical and legal implications, and the future of cloning.
Imarisio, S. et al. (2008). Huntington?s disease: from pathology and genetics to potential therapies. Biochem. J. 412, 191-209 [DOI: 10.1042/BJ20071619] [PubMed: 18466116].
General review of the disease caused by a trinucleotide expansion.