Instrumental Methods for the Analysis of Bioactive Molecules

Guddadarangavvanahally K. Jayprakasha, Bhimanagouda S. Patil, and Federica Pellati

ACS Symposium Series

Provides a comprehensive review of cutting-edge research techniques in the field of biologically active compounds derived from plants and food
Focuses on advanced instrumental methods for the identification and quantification of bioactive compounds
Provides and integrated approach to address the separation, identification, quantification, and chemistry of instrumental analysis

Instrumental Methods for the Analysis of Bioactive Molecules

Guddadarangavvanahally K. Jayprakasha, Bhimanagouda S. Patil, and Federica Pellati

Description

The main purpose of this book is to discuss cutting-edge research techniques in the field of biologically active compounds derived from plants and foods, and in particular to describe advanced instrumental methods for the identification and quantification of these important compounds. Natural products are used worldwide to prevent and treat various diseases. The efficacy and safety of natural products require the development and application of reliable analytical methods to regulate and control usage. Because of their complex chemical compositions, natural products often require separation techniques for reliable analysis of their constituents. This book provides an integrated approach to address the separation, identification, quantification and chemistry of natural products for their analysis through various instrumental analyses.

This book is divided into four sections: 1) Sample Preparation, Separation and Identification 2) Identification by LC-MS and LC-NMR 3) Identification by GC, GC-MS, LC-GC and Spectroscopic Methods and 4). Metabolomic Fingerprinting. The first section covers sample preparation, extraction and rapid flash separation techniques, rapid LC analysis with bioassays and hyphenations to identify active constituents. The second section covers chromatographic separation by LC for active constituents, identification by mass spectra, and LC-NMR for the characterization of specific constituents. The third section covers separation of volatile constituents by GC and GC-MS. In recent years, analysis of volatiles using headspace has become more sensitive and popular, as it does not alter the original composition of volatile chemicals. The fourth section focuses on metabolic fingerprinting techniques. Metabolomics involves the study of small molecules from cells, plants, foods, tissues, organisms, or other biological tissues. Metabolic fingerprinting compares patterns, also called signatures or fingerprints of metabolites; these patterns change in response to external stimuli either in different plant varieties or species, or in response to adulteration, toxins, drug exposure, environmental or genetic alterations.

This book comprehensively provides a current review that will be useful for scientists, researchers, teachers, and engineers in general, and is particularly useful for chemists, biochemists, chemical engineers, biochemical engineers, and others in chemistry-related fields.

Instrumental Methods for the Analysis of Bioactive Molecules

Guddadarangavvanahally K. Jayprakasha, Bhimanagouda S. Patil, and Federica Pellati

ACS Symposium Series

Preface
Sample Preparation, Separation, and Identification
1. Influence of Postharvest Storage, Processing, and Extraction Methods on the Analysis of Phenolic Phytochemicals
2. Innovative Methods for the Extraction and Chromatographic Analysis of Honey Bee Products
3. Separation and Identification of Cucurbitane-Type Triterpenoids from Bitter Melon
4. Fast Analysis of Bioactive Compounds by Reverse Phase Liquid Chromatography
5. Chromatography Combined with Bioassays and Other Hyphenations—The Direct Link to the Compound Indicating the Effect
Identification by LC-MS and LC-NMR
6. Standardization of Analytical Methodology and Procedures for Purity Assessment of Small Molecules in Drug Discovery
7. Biofunctional Properties of Melia azedarach Extracts
8. Characterization of Sambucus nigra L. Infusions Using Capillary Electrophoresis and Liquid Chromatography-Tandem Mass Spectrometry
9. Flavonoid C-glycosides in Citrus Juices from Southern Italy: Distribution and Influence on the Antioxidant Activity
10. Characterization of Pomegranate's Health Benefiting Bioactive Compounds, Taste, Color, and Post-Harvest Fruit Quality by Studying a Wide Collection of Diverse Accessions
11. Application of HPLC—SPE—NMR in Characterization of Bioactive Natural Compounds
Identification by GC, GC-MS, LC-GC, and Spectroscopic Methods
12. Headspace and Solid-Phase Microextraction Methods for the Identification of Volatile Flavor Compounds in Citrus Fruits
13. GC and On-line LC-GC: Useful Tools for the Qualitative and Quantitative Analysis of Phytosterols and Their Esters
14. Unraveling Food Aroma: Methods for Odor Active Compounds Analysis
15. Quantification of Health-Promoting Compounds by Quantitative 1HNMR Spectroscopy
16. Meat Freshness: Peroxynitrite's Oxidative Role, Its Natural Scavengers, and New Measuring Tools .
17. Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy
Metabolomic Fingerprinting
18. Metabolic Fingerprinting of the Lycopodiales Species for Chemotaxonomy and Quality Control
19. New Approaches in Metabolic Fingerprinting: Improved Extraction Method and Automatic Reduction of NMR Spectra to Essential Data

Instrumental Methods for the Analysis of Bioactive Molecules

Guddadarangavvanahally K. Jayprakasha, Bhimanagouda S. Patil, and Federica Pellati

ACS Symposium Series

Author Information

Guddadarangavvanahally K. Jayprakasha, Research Professor, Texas A&M University, Bhimanagouda S. Patil, Professor and Director of the Vegetable and Fruit Improvement Center, Texas A&M University, and Federica Pellati, Assistant Professor in Medicinal Chemistry, Department of Life Sciences, UNIMORE

Dr. G. K. Jayaprakasha is working as Research Professor at Texas A&M University with 24 years of research and 3 years of industrial experience.

Dr. Bhimu Patil is a Professor and Director of the Vegetable and Fruit Improvement Center at Texas A&M University and has published more than 150 peer-reviewed papers. He has been actively involved in both research and educational activities related to global "Foods for Health. "

Dr. Federica Pellati is an Assistant Professor in Medicinal Chemistry, Dept. Life Sci., UNIMORE, Italy. She is the Coordinator of International Relations and Advisor of the 2nd ACS/AGFD International Student Chapter.

Contributors:

Yingjian Lu, Devanand Luthria, Jose Luis Perez, Gislaine C. Nogueira, Mauricio A. Rostagno, M. Thereza M. S. Gomes, M. Angela A. Meireles, Gertrud E. Morlock, Aránzazu Marín, Gary Sharman, Marie Burgess, Christopher Reutter, Alfonso Espada, N. G. Ntalli, P. Caboni, Petra Cesla, Petra Dinisová, Jana Vánová, Lenka Ceslová, and Jan Fischer, Davide Barreca, Ersilia Bellocco, Ugo Leuzzi, Giuseppe Gattuso, Lior Rubinovich, Doron Holland, Rachel Amir, Chia-Chuan Chang, Shoei-Sheng Lee, Priyanka R. Chaudhary, Rebecca Esche, Birgit Scholz, Karl-Heinz Engel, Henryk Jelen, Alina Vasilescu, Alis Vezeanu, Ying Liu, Ioana S. Hosu, R. Mark Worden, Serban F. Peteu, Timothy J. Anderson, Yongfeng Ai, Roger W. Jones, Robert S. Houk, Jay-lin Jane, Yinsheng Zhao, Diane F. Birt, John F. McClelland, hee-Yan Choo, NorShahidah Sahidan, A. Latiff, Jan Schripsema, Marianna A. Lemos, Marcilene F. V. Dianin, Dalessandro S. Vianna, Denise S. Dagnino