Preface
List of Protocols
Abbreviations
1. Introduction to the principles of flow cytometry, Nigel P. Carter and Michael G. Ormerod
2. Fluorescence and fluorochromes, Michael G. Ormerod
3. Preparing suspensions of single cells, Michael G. Ormerod
4. Flow sorting, Nigel P. Carter and Michael G. Ormerod
5. Immunofluorescence of surface markers, Michael R. Loken, Cherie I. Green, and Denise A. Wells
6. Analysis of DNA - general methods, Michael G. Ormerod
7. Further clinical applications, Terry Hoy, et al.
8. Quality assurance in the clinical laboratory, Jan W. Gratama and David Barnett
9. Measurement of cytoplasmic and nuclear antigens, Jorgen K. Larsen
10. Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method, George D. Wilson
11. Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-ethidium bromide method, Michael G. Ormerod and Martin Poot
12. Chromosome analysis and sorting by flow cytometry, Derek C. Davies, Simon P. Monard, and Bryan D. Young
13. Intracellular ionised calcium, magnesium, membrane potential and pH, Peter S. Rabinovitch and Carl H. June
14. Flow cytometry in the study of apoptosis, Michael G. Ormerod
15. Further applications to cell biology, Michael G. Ormerod
A1. Safety Procedures
A2. Flow cytometers and software
A3. List of suppliers
Index